|
KMID : 0368420040470010027
|
|
Journal of Plant Biology
2004 Volume.47 No. 1 p.27 ~ p.32
|
|
|
Possible involvement of Ca2+-dependent protein kinases in spore germination of the fernOsmunda Japonica
|
|
Kamachi Hiroyuki
Noguchi Munenori
Inoue Hiroshi
|
|
|
Abstract
|
|
|
|
Fern gametophyte is a good model system to investigate signal transduction in plant cells. In this work, we examined whether CDPKs are involved in the mechanisms of spore germination of the fernOsmunda japonica. A protein extract from the spores included four CDPK isoforms with relative molecular weights of 56, 53, 49, and 47 kDa, as detected by immunoblot analysis, and they showed CDPK-like activities, as detected by in-gel protein-kinase assay. It was also found that the inhibitors effective on CDPKs, such as a general protein kinase inhibitor, K252a, and a calmodulin antagonist, W-7, largely suppressed the spore germination, and that many proteins of the spores were phosphorylated in vivo in a calcium dependent manner in the period when the spores require external Ca2+ for the germination. Furthermore, we showed that Sr2+ and Mn2+, which could substitute for Ca2+ in the spore germination, were also able to activate theOsmunda CDPKs. From these results, we concluded that CDPKs would participate in the spore germination ofO. japonica.
|
|
|
KEYWORD
|
|
|
CDPK, fern gametophyte, Osmunda japonica, spore germination
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|